Karam Aboudehen, PhD

Assistant Professor, Division of Molecular Medicine

Karam Aboudehen

Contact Info

karam@umn.edu

Lab Phone 612-625-0530

Mailing Address:
Genetics Medicine Office
MMC 485 Mayo
8485 (Campus Delivery Code)
420 Delaware
Minneapolis, MN 55455

Lab Address:
Molecular Medicine Lab
Molecular and Cellular Biology Bldg (MCB)
420 Washington Avenue SE, Suite 5-148
Minneapolis, MN 55455-0372

Assistant Professor, Division of Molecular Medicine


PhD, Human Genetics, Tulane University, New Orleans, LA

Summary

A skilled investigator with research experience in animal disease models.

Special Skills

  • Cross-linking Immunoprecipitation of endogenous RNA/protein complexes in-vivo (CLIP)
  • Chromatin IP and microarrays/Sequencing data processing and Bioinformatics analysis
  • Molecular cloning
  • Fluorescence microscopy
  • Immunoassays
  • Tissue and bacterial culture

Professional Achievements

  • Identification of pathologic miRNAs and LncRNA in Polycystic kidney disease (PKD). Specifically identified direct miRNA targets in mIR-17 inducible kidney cell line.
  • Led a team of intra-departmental collaborators on project investigating the abnormal metabolic pathways in a PKD mouse model.
  • Two first authorship manuscripts.

Research

Research Summary/Interests

Investigate the role of non-coding RNA in the pathogenesis of Polycystic Kidney Disease (PKD). Specifically interested in identifying pathologic non-coding RNA that has potential in being therapeutic targets in PKD.

Publications

  • Aboudhene, K., Kim, M.S., Mitsche, M., Garland, K., Anderson, N., McDonald, J., Patel, V., Xie, Y., Debose-Boyd, R., and Igarashi P. Multilevel regulation of cholesterol metabolism by transcription factor HNF-1?. Submitted to JASN.
  • Aboudehen, K., Hilliard, S., Saifudeen, Z., and El-Dahr, S. S. Mechanisms of p53 Activation and Physiological Relevance in the developing kidney. Am J Physiol Renal Physiol. 302: F928-40, 2012.
  • Hilliard, S. , Aboudehen, K. , Yao, X. , and El-Dahr, S. S. Tight regulation of p53 activity by Mdm2 is required for ureteric bud growth and branching. Dev Biol. 253: 354-366, 2011.