Douglas G. Mashek, PhD

Associate Professor of BMBB and Medicine (Joint Appointment), Biochemistry, Molecular Biology, and Biophysics

Douglas G. Mashek

Contact Info

Mailing Address:
6-155 Jackson Hall
1214A
321 Church St. SE
Minneapolis, MN 55455

Lab Address:
420 Washington Avenue SE
MCB 7-160
Minneapolis, MN 55455

Associate Professor of BMBB and Medicine (Joint Appointment), Biochemistry, Molecular Biology, and Biophysics

Head, Division of Metabolic and Systems Biology


Postdoctoral Fellowship, University of North Carolina-Chapel Hill

PhD, University of Wisconsin, Madison, WI

MS, Michigan State, East Lansing, MI

BS, Iowa State University, Ames, IA

Summary

Awards & Recognition

  • CFANS Distinguished Teaching Award
  • American Society for Nutrition E.L.R. Stokstad Award
  • American Diabetes Association Basic Science Award
  • NIH/NIDDK National Research Service Award
  • American Diabetes Association Junior Faculty Award
  • Dannon Nutrition Leadership Institute
  • Novo Nordisk Diabetes Innovation Award

Professional Associations

  • American Diabetes Association
  • American Society for Biochemistry and Molecular Biology
  • American Society for Nutrition
  • The Obesity Society

Research

Research Summary/Interests

Research in the Mashek laboratory focuses on the relationship between lipid metabolism and disease development. A primary emphasis is on studies involving lipid droplet biology in the context of non-alcoholic fatty liver disease, Type 2 Diabetes, cancer and aging. Our studies span a range of disciplines that ultimately link nutrition, biochemistry and physiology - in other words, we study metabolism. An important goal of the laboratory is to do research that helps us identify detailed mechanisms of disease using animal and cell models and then translate these findings to the clinic. Ultimately, we hope that by advancing our knowledge into the basic metabolism of nutrients we will come closer to understanding how metabolic diseases develop and, therefore, will be better equipped to identify new preventions or treatments.

Publications

  • Franklin MP, Sathyanarayan S, Mashek DG. Acyl-CoA Thioesterase 1 (ACOT1) regulates PPAR? to couple fatty acid flux with oxidative capacity during fasting. Diabetes (in press)
  • Schulze RJ, Sathyanarayan A, Mashek DG. Breaking fat: the regulation and mechanisms of lipophagy. BBA - Molecular and Cell Biology of Lipids (in press)
  • Shepherd SO, Strauss JA, Wang Q, Dube JJ, Goodpaster BH, Mashek DG, Chow LS. Training alters the distribution of perilipin proteins in muscle following acute free fatty acid exposure. Journal of Physiology (in press)
  • Chow LS, Mashek DG, Wang Q, Shepherd SO, Goodpaster BH, Dube JJ. Effect of acute physiological free fatty acid elevation in the context of hyperinsulinemia on fiber type specific IMCL accumulation. Journal of Applied Physiology (in press)
  • Sathyanarayan S, Mashek MT, Mashek DG. ATGL promotes autophagy/lipophagy via SIRT1 to control hepatic lipid droplet catabolism. Cell Reports 2017;19(1):1-9.
  • Kamarajugadda S, Becker JR, Hanse EA, Mashek DG, Mashek MT, Hendrickson AM, Mullany LK, Albrecht JH. Cyclin D1 represses peroxisome proliferator-activated receptor alpha and inhibits fatty acid oxidation. Oncotarget 2016;7(30):47674-47686.
  • Zhang W, Bu SY, Mashek MT, O-Sullivan I, Sabai Z, Khan SA, Ilkayeva O, Newgard CB, Mashek DG*, Unterman TG*. Integrated regulation of hepatic lipid and glucose metabolism by adipose triacylglycerol lipase and FoxO proteins. Cell Reports 2016;5(2):349-359. *Co-corresponding authors
  • Bowman TA, O’Keeffe KR, D’Aquila T, Yan QW, Griffin JD, Killion EA, Mashek DG, Buhman KK, Greenberg AS. Acyl CoA synthetase 5 (ACSL5) ablation in mice increases energy expenditure and insulin sensitivity and delays fat absorption. Molecular Metabolism 2016;5(3):210-220.
  • Mashek DG, Khan SA, Sathyanarayan A, Ploeger JM, Franklin MP. Hepatic lipid droplet biology: Getting to the root of fatty liver. Hepatology 2015;62(3):964-967.
  • Khan SA, Wollaston-Hayden EE, Markowski TW, Higgins L, Mashek DG. Quantitative analysis of the murine lipid droplet-associated proteome during diet-induced hepatic steatosis. Journal of Lipid Research 2015;56(12):2260-2272.
  • Khan SA, Sathyanarayan S, Mashek MT, Ong KT, Wollaston-Hayden EE, Mashek DG. ATGL-catalyzed lipolysis regulates SIRT1 to control PGC-1?/PPAR-? signaling. Diabetes 2015;64:418–426.
  • Zhang Y, Guo H, Deis JA, Mashek MG, Zhao M, Ariyakumar D, Armien AG, Bernlohr DA, Mashek DG, Chen X. Lipocalin 2 regulates brown fat activation via a non-adrenergic activation mechanism. Journal of Biological Chemistry 2014;289(32):22063-77.
  • Chow LS, Mashek DG, Austin E, Eberly LE, Persson XM, Mashek MT, Seaquist, ER, Jensen MD. Training status diverges muscle diacylglycerol accumulation during free fatty acid elevation. American Journal of Physiology Endocrinology and Metabolism 2014;307(1):E124-31.
  • Ong KT, Mashek MT, Davidson NO, Mashek DG. Hepatic ATGL mediates PPAR-? signaling and fatty acid channeling through an L-FABP independent mechanism. Journal of Lipid Research 2014;55(5):808-15.

Teaching

Courses

BioC 8006 - Biochemistry: Metabolism and Control